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Table 4 PCR amplification of Wuchereria bancrofti DNA in pools of laboratory-reared uninfected mosquitoes spiked with microfilariae

From: PCR and dissection as tools to monitor filarial infection of Aedes polynesiensismosquitoes in French Polynesia

Pool size Control testing 191 bp band 225 bp band Résults
0 Mf spiked controls + (n = 3/3) + (n = 3/3) + (n = 3/3)
  Negative control - (n = 1) + (n = 1) - (n = 1)
20 Mf spiked controls + (n = 3/3) + (n = 3/3) + (n = 3/3)
  Negative control - (n = 1) + (n = 1) - (n = 1)
30 Mf spiked controls + (n = 3/3) + (n = 3/3) + (n = 3/3)
  Negative control - (n = 1) + (n = 1) - (n = 1)
50 Mf spiked controls + (n = 3/3) + (n = 3/3) + (n = 3/3)
  Negative control - (n = 1) + (n = 1) - (n = 1)
  1. * DNA extracted according the B method; sample fraction used 1/240e
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Filaria Journal

ISSN: 1475-2883